Fig. 4.

Late-life EOD fasting improves glucose handling and clearance capacity. a Average 4–6-h fasting blood glucose levels (mg/dL) prior to the start of the glucose tolerance test (GTT) taken on adjacent fasted and fed days in male (left) and female (right) Chow AL and Chow EOD groups (n = 5–6 mice per sex per group per day). b Average blood glucose levels (mg/dL) at time points between 0 and 120 min following an intraperitoneal injection of glucose (2 g of glucose/kg of body weight) from the GTT being performed on adjacent fasted and fed days in the male (left) and female (right) Chow AL and Chow EOD groups (n = 5–6 mice per sex per group per day). The inset shows the AUC of 0–120 min post-injection blood glucose levels in the male (left) and female (right) Chow AL and Chow EOD groups. b HbA1c levels in the male Chow AL (n = 6) and Chow EOD groups (n = 5). The figures (a, b) depict the mean with error bars (± SEM). The asterisks indicate the significant difference between the same-sex Chow AL and Chow EOD groups. *p < 0.05 and **p < 0.01. See also Supplemental Figure 4