Table 3.

Estimated effects and coefficients of the response (cell yields)

Term	Effect	Coefficient	T	p-value
Constant	762,938	146.00	0.000
A	− 23,625	− 11,812	− 2.27	0.151
B	− 12,375	− 6187	− 1.19	0.356
C	− 75,375	− 37,687	− 7.24	0.019
D	− 78,625	− 39,313	− 7.55	0.017
E	6375	3188	0.61	0.603
F	72,375	36,187	6.95	0.020
AB	− 53,875	− 26,938	− 5.18	0.035
AC	32,125	16,062	3.09	0.091
AD	8875	4437	0.85	0.484
AE	− 95,125	− 47,563	− 9.14	0.012
AF	10,875	5437	1.04	0.406
BD	25,125	12,563	2.41	0.137
BF	48,125	24,063	4.62	0.044
ABD	− 15,375	− 7688	− 1.48	0.278
ABF	− 59,375	− 29,687	− 5.70	0.029
Center point	–	57,604	− 4.40	0.048

S = 20,816.7, R² = 99.46%, R² (adjusted) = 95.13%, Significant at p-value < 0.05. A (Bacto™ soytone), B (Bacto™ yeast extract), C (vitamin C); D (vitamin B12), E (SITE liquid media supplement), and F (recombinant human epidermal growth factor, rEGF) were the factors (serum substitutes) used in a 2^6–2 fractional factorial design