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. 2021 Oct 5;11:19798. doi: 10.1038/s41598-021-99366-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The ΔCt values of Stat3 expression target gene normalized by different combinations of the three best candidate housekeeping genes (Actb, Hprt and Ppia) (A) Stat3/Actb, target expression normalized by Actb; (B) Stat3/Hprt, target expression normalized by Hprt; (C) Stat3/Ppia, target expression normalized by Ppia; (D) Stat3/Actb + Hprt, target expression normalized by Actb + Hprt; (E) Stat3/Actb + Ppia, target expression normalized by Actb + Ppia; (F) Stat3/Hprt + Ppia, target expression normalized by Hprt + Ppia, (G) Stat3/Actb + Hprt + Ppia target expression normalized by Actb + Hprt + Ppia. The median values are represented as horizontal lines and the error bars represent interquartile ranges. CY, cystic; NC, noncystic; HT, haploinsufficient; WT, wild type; SC, severely cystic phenotype; CO, control for the severely cystic phenotype. *p < 0.05 by Kruskal–Wallis with Dunn’s post-hoc test, followed by FDR correction.