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. 2021 Sep 29;53(9):1437–1448. doi: 10.1038/s12276-021-00678-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, b Overall scheme showing the processes for constructing the human naïve scFv antibody library (a) and screening for the anti-ET_A antibody using the phage display system (b). c Amino acid sequences of the framework and complementarity-determining regions in AG8. d β-Arrestin Tango recruitment assay using bombesin and ET-1 in cells that express ET_A/ET_B receptors. e β-Arrestin Tango recruitment assay using M13 phage particles displaying AG8 scFv. The error bars show the mean ± standard deviation values; *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 vs. control.