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. 2021 Sep 29;53(9):1437–1448. doi: 10.1038/s12276-021-00678-9

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© The Author(s) 2021

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Fig. 3 — a SDS–PAGE gel showing the band for the purified scAb AG8. b, c Calcium flux assay with the scAb AG8 in CHO-K1 cells expressing hET_A (b) and in HT-29 cells (c). d SDS–PAGE showing purified AG8 IgG. e ELISA showing the cross-species binding property of AG8 IgG to human ET_A and mouse ET_A.