Fig. 1.

Cardiac remodeling in response to 1 week of isoproterenol (ISO) by age and sex. a Heart weight normalized to tibia length (HW/TL) differed by age (juvenile < adult < aged), sex, condition, and the interactions age × sex and age × condition. b LV weight normalized to TL (LV/TL) differed by age (juvenile < adult < aged), condition, and the interactions age × sex and age × condition. c HW normalized to BW (HW/BW) differed by age (juvenile > adult = aged), condition, and the interactions age × sex and age × condition D) LV weight normalized to BW (LV/BW) differed by condition, n = 6/group. e Representative images (× 40 magnification) from WGA-FITC staining of LV sections. f Cardiomyocyte size differed by age (juvenile < adult < age), sex, condition, and the interaction age × condition. g Representative images (× 20 magnification) from Trichrome staining of LV sections. h Fibrosis differed by age (juvenile = adult < aged), condition, sex, and the interaction age × condition, n = 3 biological replicates, 6 technical replicates. *P < 0.05 as assessed by 3-way ANOVA and Fisher’s post hoc test to compare ISO and CON within age and sex. Data are presented as means ± SEM