Fig. 3.

KHDRBS1 is a targeted binding molecule of miR-200b-3p. A Targetscan predicts the binding sites of miR-200b-3p and KHDRBS1. B Dual-luciferase reporter assay verified the targeted binding of miR-200b-3p and KHDRBS1. (^* vs. MC; ^***P < 0.001). C qRT-PCR analysis of KHDRBS1 expression in each group rats. (n = 10, ^# vs. Control; ^{^} vs. Model+agomiR-NC; ^§ vs. Model+antagomir-NC. ^§§§P < 0.001; ^***P < 0.001; ^###P < 0.001). D Western blot analysis of KHDRBS1 expression in each group rats. E The signal intensities of KHDRBS1 were quantified. (n = 10, ^# vs. Control; ^{^} vs. Model+agomiR-NC; ^§ vs. Model+antagomiR-NC. ^§§§P < 0.001; ^***P < 0.001; ^###P < 0.001). Group: Control group; Model group; Model+agomiR-NC group; Model+agomiR group; Model+antagomiR-NC group; Model+antagomiR group. qRT-PCR quantitative reverse transcription polymerase chain reaction, KHDRBS1 KH RNA binding domain containing, signal transduction associated 1