Table 3.
Effect of different ratios of cellulose and starch (C:S) on ruminal OBCFA synthesis (mg/g DM) during 24 h of incubation in vitro.
| Time (h) | iso-C15:0 | anteiso-C15:0 | C15:0 | iso-C16:0 | iso-C17:0 | anteiso-C17:0 | C17:0 | TC15a | TC17b | TIFAc | TAFAd | TOCFAe | TBCFAf | OBCFAg | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| C:S = 0:100h | 6 | 0.22 | 0.54 | 0.56 | 0.31 | 0.31 | 0.37 | 0.35 | 1.31 | 1.02 | 0.84 | 0.90 | 0.90 | 1.74 | 2.64 |
| 12 | 0.23 | 0.58 | 0.51 | 0.33 | 0.36 | 0.38 | 0.27 | 1.29 | 1.00 | 0.91 | 0.96 | 0.78 | 1.87 | 2.65 | |
| 18 | 0.24 | 0.60 | 0.55 | 0.38 | 0.39 | 0.46 | 0.31 | 1.39 | 1.17 | 1.01 | 1.06 | 0.87 | 2.07 | 2.93 | |
| 24 | 0.31 | 0.76 | 0.69 | 0.48 | 0.47 | 0.80 | 0.43 | 1.75 | 1.71 | 1.26 | 1.56 | 1.12 | 2.82 | 3.94 | |
| C:S = 25:75 | 6 | 0.19 | 0.43 | 0.33 | 0.25 | 0.32 | 0.30 | 0.32 | 0.95 | 0.94 | 0.76 | 0.73 | 0.65 | 1.49 | 2.14 |
| 12 | 0.21 | 0.56 | 0.42 | 0.26 | 0.33 | 0.35 | 0.25 | 1.19 | 0.92 | 0.80 | 0.90 | 0.66 | 1.71 | 2.37 | |
| 18 | 0.24 | 0.64 | 0.62 | 0.34 | 0.41 | 0.49 | 0.33 | 1.50 | 1.24 | 0.99 | 1.13 | 0.95 | 2.13 | 3.07 | |
| 24 | 0.30 | 0.75 | 0.59 | 0.42 | 0.45 | 0.64 | 0.40 | 1.64 | 1.48 | 1.17 | 1.39 | 0.99 | 2.56 | 3.55 | |
| C:S = 50:50 | 6 | 0.14 | 0.29 | 0.34 | 0.17 | 0.26 | 0.24 | 0.22 | 0.76 | 0.72 | 0.57 | 0.53 | 0.56 | 1.09 | 1.65 |
| 12 | 0.21 | 0.57 | 0.54 | 0.26 | 0.35 | 0.44 | 0.24 | 1.32 | 1.03 | 0.82 | 1.01 | 0.79 | 1.83 | 2.62 | |
| 18 | 0.25 | 0.59 | 0.61 | 0.23 | 0.35 | 0.40 | 0.23 | 1.45 | 0.98 | 0.83 | 0.98 | 0.85 | 1.81 | 2.66 | |
| 24 | 0.33 | 0.76 | 0.61 | 0.38 | 0.40 | 0.83 | 0.29 | 1.70 | 1.52 | 1.11 | 1.58 | 0.91 | 2.69 | 3.60 | |
| C:S = 75:25 | 6 | 0.18 | 0.40 | 0.32 | 0.18 | 0.32 | 0.25 | 0.23 | 0.91 | 0.81 | 0.68 | 0.66 | 0.55 | 1.34 | 1.89 |
| 12 | 0.28 | 0.71 | 1.03 | 0.30 | 0.35 | 0.34 | 0.27 | 2.01 | 0.95 | 0.92 | 1.04 | 1.30 | 1.96 | 3.26 | |
| 18 | 0.37 | 0.76 | 1.10 | 0.25 | 0.41 | 0.31 | 0.31 | 2.24 | 1.04 | 1.03 | 1.08 | 1.42 | 2.11 | 3.52 | |
| 24 | 0.37 | 0.82 | 1.04 | 0.33 | 0.44 | 0.35 | 0.44 | 2.24 | 1.24 | 1.15 | 1.18 | 1.48 | 2.32 | 3.81 | |
| C:S = 100:0 | 6 | 0.14 | 0.31 | 0.37 | 0.11 | 0.32 | 0.26 | 0.24 | 0.82 | 0.81 | 0.57 | 0.57 | 0.61 | 1.13 | 1.74 |
| 12 | 0.23 | 0.63 | 0.94 | 0.18 | 0.33 | 0.28 | 0.29 | 1.80 | 0.90 | 0.74 | 0.91 | 1.23 | 1.64 | 2.87 | |
| 18 | 0.29 | 0.65 | 1.15 | 0.15 | 0.35 | 0.25 | 0.30 | 2.09 | 0.91 | 0.79 | 0.91 | 1.45 | 1.70 | 3.15 | |
| 24 | 0.29 | 0.64 | 1.25 | 0.16 | 0.38 | 0.26 | 0.32 | 2.18 | 0.95 | 0.83 | 0.90 | 1.56 | 1.72 | 3.28 | |
| SEM | 0.015 | 0.034 | 0.049 | 0.016 | 0.018 | 0.025 | 0.019 | 0.080 | 0.037 | 0.038 | 0.045 | 0.057 | 0.073 | 0.110 | |
| C:S | <0.0001 | <0.0001 | <0.00011 | <0.0001 | 0.0011 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | |
| P | Time | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 |
| C:S × Time | 0.0003 | 0.001 | <0.0001 | <0.0001 | 0.13 | <0.0001 | 0.0006 | <0.0001 | <0.0001 | 0.01 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | |
| Polynomial contrast-C:S | Linear | 0.11 | 0.44 | <0.0001 | <0.0001 | 0.02 | <0.0001 | 0.0002 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | 0.22 |
| Quadratic | 0.37 | 0.78 | <0.0001 | 0.03 | 0.54 | 0.0002 | 0.001 | 0.001 | 0.54 | 0.32 | 0.02 | <0.0001 | 0.05 | 0.04 | |
| Polynomial contrast-Time | Linear | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | <0.0001 |
| Quadratic | 0.27 | 0.0004 | <0.0001 | 0.09 | 0.75 | <0.0001 | <0.0001 | <0.0001 | <0.0001 | 0.67 | 0.95 | 0.0005 | 0.79 | 0.08 |
TC15 = iso-C15:0 + anteiso-C15:0 + C15:0;
TC17 = iso-C17:0 + anteiso-C17:0 + C17:0;
TIFA = iso-C15:0 + iso-C16:0 + iso-C17:0;
TAFA = anteiso-C15:0 + anteiso-C17:0;
TOCFA = C15:0 + C17:0;
TBCFA = iso-C15:0 + iso-C16:0 + iso-C17:0 + anteiso-C15:0 + anteiso-C17:0;
OBCFA = iso-C15:0 + anteiso-C15:0 + C15:0 + iso-C16:0 + iso-C17:0 + anteiso-C17:0 + C17:0;
C:S = cellulose:starch.