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. 2021 Sep 22;12:666594. doi: 10.3389/fimmu.2021.666594

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Copyright © 2021 Gao, Yue and Xiong

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Albumin-binding ability of ABD-VP1vaccine. (A) SDS-PAGE and (B) western blot analysis of the recombinant ABD-VP1 and VP1 proteins. (C) The binding abilities of ABD-VP1 and VP1 proteins to albumins were detected by ELISA assays. (D) Serum complex of ABD-VP1 with albumin was detected by ELISA assays. (E) The serum half-lives of ABD-VP1 and VP1 vaccines. VP1 and ABD-VP1 proteins were injected subcutaneously into mice, sera were taken at indicated time points for VP1 amount detection by ELISA assays. The level of serum VP1 protein at 5 min after injection was uniformly quantified as 100%. Individual experiment was repeated 3 times. *P < 0.05; **P < 0.01; ***P < 0.001.