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. 2021 Sep 22;12:747010. doi: 10.3389/fphar.2021.747010

FIGURE 4.

FIGURE 4

Luteolin inhibits the NF-κB signaling pathway. (A) Western blotting of pro IL-1β, NLRP3, Casp-1 p45, ASC, and GAPDH in BMDMs treated with apigenin, esculetin, gomisin N, schisanhenol, schisandrin A, schisandrin B, anwulignan, schisantherin A, schisandrin B, specnuezhenide, schisandrin, luteolin, quinic acid, and curcumenol (40 μM) for 4 h and then stimulated with LPS (50 ng/ml) for 1 h. (B,C) ELISA of TNF-α (B) and IL-6 (C) in SN from samples described in A. (D) Western blotting of pro IL-1β, NLRP3, Casp-1 p45, ASC, and GAPDH in BMDMs treated with luteolin for 4 h and then stimulated with LPS (50 ng/ml) for 1 h. (E,F) ELISA of TNF-α (E) and IL-6 (F) in SN from samples described in D. GAPDH served as a loading control. Data are represented as the mean ± SD using biological samples.