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Potentiation of the AR by SRC1 requires the glutamine-rich region, residues 1053 to 1123. (A) COS-1 cells were transfected with 50 or 200 ng of each deletion mutant of SRC1. CAT activity was measured in the absence (black bars) or presence (gray bars) of 10 nM mibolerone. (B) Alignment of the p160 coactivator proteins in the glutamine-rich region. Residues conserved across all three proteins are boxed.
