Table 4.
Antagonism assays using selected combinations of yeast and filamentous fungal strains, challenged with environmental stressors.
| Fungal strains | M. perniciosa CBS 441.80 vs | M. perniciosa CBS 442.80 vs | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Yeast strains | M. guilliermondii | W. anomalus | S. cerevisiae | M. guilliermondii | W. anomalus | S. cerevisiae | ||||
| #1015 | #1105 | #1112 | CAT1 | PE2 | #1015 | #1105 | #1112 | CAT1 | PE2 | |
| Standard assay | 2 | 2 | 2 | 1 | 2 | 2 | 2 | 2 | 2 | 2 |
| 16°C | 2 | 2 | 2 | 0 | 2 | 0 | 2 | 2 | 0 | 2 |
| 25°C | 2 | 2 | 2 | 0 | 2 | 0 | 2 | 2 | 0 | 2 |
| Starved inoculum | 2 | 2 | 2 | 1 | 2 | 2 | 2 | 2 | 2 | 2 |
| Aged inoculum | 2 | 2 | 2 | 1 | 2 | 2 | 2 | 2 | 1 | 2 |
| Stationary phase inoculum | 2 | 2 | 2 | 0 | 2 | 2 | 2 | 2 | 1 | 2 |
Temperatures of 16°C or 25°C; yeasts starved for 3days in sterile water or aged for 10 days in solid medium at room temperature; or yeast inoculum from liquid medium from 3-day-old stationary phase culture. The inhibitory effect was rated according to Figure 1. Identical results were obtained in three independent replicates.