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. 2021 Sep 22;12:671152. doi: 10.3389/fphar.2021.671152

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Copyright © 2021 Fu, Xiao, Tian, Liu, Xu, Yang, Hu, Zhou, Fang, Gao, Zhang, Mu, Zhang, Hu, Huang, Chen and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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JY5 significantly alleviates hepatic inflammatory injury and collagen deposition in CCl₄-induced rat liver fibrosis. Serum levels of ALT (A) and AST (B) were measured in each group. (C) H&E staining (100×), SR staining (100×), Masson staining (100×) and IHC staining of Col-I (100×), and semi-quantitative analysis (D) of collagen disposition (%) in SR-stained liver sections (E) Hyp content in wet liver tissue was detected by alkaline hydrolysis. (F) The mRNA levels of Col-I in liver tissue were analyzed by qPCR. (G) quantitative analysis of immunohistochemical staining for Col-I. **p < 0.01, ***p < 0.001 vs the control group, ^# p < 0.05, ^## p < 0.01 vs the CCl₄ group. Oil: control group.