FIGURE 6.

JY5 may ameliorate liver fibrosis by inhibiting the Notch signaling pathway in vivo. (A) In the CCl₄-induced rat liver fibrosis experiment, the mRNA levels of Jagged1, Jagged2, Notch2, Notch3, Notch4 and RBP-κB were measured by qPCR. Western blotting (B) and quantitative analysis (C) of Jagged1, Notch2, Notch3 and RBP-κB protein. (D) In the CCl₄-induced liver fibrosis mice experiment, the mRNA levels of Dll1, Jagged1, Notch2, Notch3, Notch4 and RBP-κB were measured by qPCR. Western blotting (E) and quantitative analysis (F) of Jagged1, Notch2, Notch4 and RBP-κB protein. (G) In the BDL-induced rat liver fibrosis experiment, the mRNA levels of Dll1, Dll4, Jagged1, Jagged2, Notch2, Notch3, Notch4 and RBP-κB were measured by qPCR. Western blotting (H) and quantitative analysis (I) of Jagged1, Notch2, Notch3 and RBP-κB protein. *p < 0.05, **p < 0.01, ***p < 0.001 vs the control group or sham group, ^# p < 0.05, ^## p < 0.01, ^### p < 0.001 vs the CCl₄ group or BDL group. Oil: control group.