Figure 1.

Transmission of Burkholderia strains from Lagria villosa adults to wheat, rice, and soybean plants. Results were obtained with diagnostic PCRs using Burkholderia-specific primers targeting the 16S rRNA and gyrB genes, or Burkholderia Lv-StB – specific primers targeting the lgaG gene. Sanger or Illumina sequencing was carried out for the obtained amplicons. The B. gladioli strains targeted by each method are indicated below the blue horizontal lines. Blue filled tiles represent a positive indication of Burkholderia presence, empty tiles indicate no sign of Burkholderia presence, and grey tiles indicate the method was not applied on that sample. Occasionally, a signal was found via PCR but sequencing was unsuccessful. All controls (N=18 for wheat and rice, respectively, N=9 for soybean) were negative across all methods.