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. 2021 Sep 22;12:715601. doi: 10.3389/fmicb.2021.715601

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Copyright © 2021 Wierz, Gaube, Klebsch, Kaltenpoth and Flórez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Symbiont transmission from beetles to leaf litter. Burkholderia 16S rRNA was detected in leaf litter samples exposed to adult L. villosa beetles (N=12) in significantly higher copy numbers than in unexposed leaves serving as a control (N=10; Wilcoxon Rank Sum test, p<0.001), which resembled quantitative PCR (qPCR) negative controls (NC). Dots within the violin plot represent individual samples and horizontal lines indicate the median. NC includes sterile water (3x) and Escherichia coli genomic DNA (2x).