Figure 2.

MS-IR is systemic and long-lasting. (A,B) 4-week-old plants were locally pretreated with 50 μM AA or Mock by syringe infiltration and infected with Hpa at 3 dat. Hpa colonization was addressed 5 dpi (8 dat) by trypan blue staining, followed by scoring of the oomycete development with the help of a stereomicroscope (see Figure 1 legend). The letters above the bars indicate statistically significant differences by chi-square tests p ≤ 0.01. (A) Local AA-IR. Hpa colonization was assessed in the pretreated and infected leaves. n = 75. (B) Systemic AA-IR. Hpa colonization was assessed in distal (non-AA/Mock treated leaves from AA/Mock locally treated plants) and infected leaves. n = 92. (C) Transgenerational IR. Hpa colonization was monitored in the direct progeny (F1) of AA or Mock-treated plants. Shown are the results of pooled infected 2.5-week-old seedlings from four lines/treatment. Results from independent lines are shown in Supplementary Figure 2. The letters above the bars indicate statistically significant differences by chi-square tests p ≤ 0.01. n = 320.