Figure 3.

MS-induced resistance is associated with a faster/stronger induction of several defense-related genes. About 2.5-week-old plants were pretreated with 50 μMAA (or Mock) +0.02% tween 20 by spray. Three dat, the seedlings were infected with Hpa. Samples for RNA extraction were collected at 0, 2, and 3 dpi. Each sample contained around 10 seedlings. X axis indicates gene expression analyzed by RT-qPCR, using specific primers and relative to Mock pretreated samples at time 0 (pre-inoculation). For each sample, the total amount of RNA was corrected using the expression of the housekeeping genes At5g25760 (UBC) and At2g28390 (SAND). The boxplot represents the interquartile range (IQR; Q3-Q1: as the distance between the median of the lower half of the data set –Q1- and highest half –Q3-, including the central 50% of the data). The horizontal line inside the boxes represents the sample median. Whiskers are drawn ± the last datapoint within 1.5 times the IQR. Replication units are shown as overlaying jittered-dot-plots. Asterisks label statistically significant differences between AA and Mock pretreated samples (p < 0.05, Student's T-test), n = 3–4.