FIGURE 6.

KDM4A silencing or inhibition attenuates TGF-β induced EMT in a SIRT1-dependent manner. (A,B) IEC-6 cells were transfected with indicated siRNAs followed by treatment with TGF-β (2 ng/ml) for 48 h. Gene expression levels were examined by qPCR and Western. (C,D) IEC-6 cells were transfected with indicated siRNAs followed by treatment with TGF-β (2 ng/ml) in the presence or absence of EX527 (10 μM) for 48 h. Gene expression levels were examined by qPCR and Western. All experiments were performed in triplicate wells and repeated three times. One representative experiment is shown. (E) A schematic model.