Figure 4.

NCR3-CAR promoted NK killing of fulvestrant-resistant cells. A. MCF7 and the ICI-R derivatives were co-cultured with the B7-H6-directed CAR-NK or the mock-transduced NK cells for 24 h. After incubation, the apoptotic cells were analyzed with annexin V-conjugated antibody and 7-AAD staining and detected by flow cytometry. B. The quantitative results of cytotoxicity assay by flow cytometry. The results are presented by the mean ± SD based on three independent experiments. Statistical significance was determined by t-test. *, P < 0.05, **, P < 0.01, ***, P < 0.001.