Figure 5.

Fulvestrant-resistant T47D derivatives expressed higher levels of cell surface B7-H6 and were sensitized to anti-B7-H6 CAR-NK. A. B7-H6 surface expressions of T47D parental and the ICI-R derivatives were assessed by flow cytometry. B. The mean fluorescent intensity obtained by flow cytometry. Results are presented as mean ± SD based on three independent experiments. Statistical significance was determined by t-test: *, P < 0.05, **, P < 0.01. C. T47D and ICI-R derivatives were co-cultured with the B7-H6-directed CAR-NK or mock-transduced NK cells for 12 h. After incubation, the apoptotic cells were analyzed with annexin V-conjugated antibody and 7-AAD staining and detected by flow cytometry. D. The quantitative results of cytotoxicity assay by flow cytometry. The results are presented by the mean ± SD based on three independent experiments. Statistical significance was determined by t-test. *, P < 0.05, **, P < 0.01, ***, P < 0.001.