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. 2021 Sep 15;11(9):4199–4219.

Figure 3.

Figure 3

Capsaicin-induced autophagy is dependent of ROS signaling in A375 cells. A. The percent change in intracellular ROS generation was evaluated after cells were treated with various concentrations of capsaicin for 1 h. Values (means ± SDs) are from no less than three independent experiments (*P<0.05, ***P<0.001). B. Cells were pretreated with or without 10 mM NAC for 1 h before exposure to 200 μM capsaicin or ethanol for 18 hours. The percentage of autophagic cells was assessed by flow cytometry, and the results are expressed as a percentage of autophagic cells. Values (means ± SDs) are from at least three independent experiments (*P<0.05, ***P<0.001). C. Cells were pretreated with or without 10 mM NAC for 1 h before exposure to capsaicin or ethanol for 24 hours. Aliquots of cell lysates were resolved by SDS-PAGE and analyzed for protein expression by Western blotting. β-actin was used as an internal loading control to monitor for equal loading. Representative images are shown from at least three independent experiments.