Figure 7.

In vivo verification for capsaicin-reduced tNOX and tNOX-depletion suppresses melanoma tumor growth. A. In a tumor-bearing mouse xenograft model, control mice were intratumorally injected with vehicle buffer and treatment group mice were intratumorally treated with 200 μg capsaicin as described in Materials and Methods. The morphology of the tumor tissues excised from tumor-bearing mice (top panel) and quantitative analysis of xenografted tumor volume during the treatment period (bottom panel) are shown. B. Tissues from two sets of tumor-bearing mice were grounded and prepared for Western blotting analysis. β-actin was used as an internal loading control to monitor for equal loading. C. The lysates of tumor tissues were immunoprecipitated with nonimmune IgG or an antibody against ULK1, and the bound proteins were detected by Western blotting with pan-acetylated lysine. D. In another mouse xenograft model, B16F10 cells transfected with either control (control-i) or tNOX (tNOX-i) shRNA were subcutaneously injected into mice. The morphology of the tumor tissues excised from tumor-bearing mice (top panel) and quantitative analysis of xenografted tumor weights from two sets of mice are shown.