FIG. 4.

Analysis of growth-regulated expression from integrated cad promoter constructs. Graphical representation of relative fold induction of stably integrated cad promoter-reporter activity throughout the growth cycle. NIH 3T3 cells were transfected with the indicated constructs, and stably transfected clones were selected with G418. Clones were serum starved for 48 h and then stimulated to grow by the addition of 10% serum to the culture medium. Cells were harvested for luciferase activity at the points indicated. Relative fold activation was calculated by normalizing the luciferase activity from serum-stimulated cells to the activity of the same clone prior to serum treatment. Data represents the average activity of one clone for each construct obtained from three independent time course experiments. Error bars represent the standard errors of the means.