Table 1.
Most frequently expressed 19-bp fragments in drug-resistant cells
| No.a | Tag | iMEFn19 cells | UACC-62n19 cells | QGY-7701R cells |
|---|---|---|---|---|
| n (%) | n (%) | n (%) | ||
| 1 | TTCTGCTCTGTCGTATCTG | 0 (0.00) | 0 (0.00) | 1,004,306 (13.87) |
| 2b | TATATCTTCACCTTTAGCT | 0 (0.00) | 0 (0.00) | 537,615 (7.43) |
| 3 | ATAAGTATGCAAGAATATG | 0 (0.00) | 0 (0.00) | 445,149 (6.15) |
| … | … | … | … | … |
| 5266c | Total | 211,364 (5.30) | 209,003 (5.46) | 6,647,889 (91.83) |
Data expressed as n (%).
a
The three most frequently expressed 19-bp fragments are listed.
b
Through a blast search with NCBI, the fragment was determined to perfectly match the PTEN gene (query cover, 100%; identify, 100%).
c
The fragments read by NGS were listed from the highest to the lowest.
From the 1st to the 5266th one, all the fragments sequenced were calculated totally and presented as n (%).