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. 2021 Sep 28;16(25):2291–2303. doi: 10.2217/nnm-2021-0080

Figure 2. . In vitro uptake of miR-125 encapsulated HA-PEI/PEG-M2 peptide by J774 murine macrophages.

Figure 2. 

(A) J774 murine macrophage cells were treated with Interleukin-4 (100 ng/ml) for 6 h, followed by treatment with Cy5-labeled HA-PEI/HA-PEG-scram peptide or HA-PEI/HA-PEG-M2 peptide nanoparticles for 2 h, 4 h, 6 h and 12 h. Representative images of cells were stained with DAPI for confocal analysis. Scale bar indicates 50 μm. (B) J774 murine macrophage cells were treated with IL-4 (100 ng/ml) for 6 h, followed by treatment with Cy5-labeled HA-PEI/HA-PEG-scram peptide or HA-PEI/HA-PEG-M2 peptide nanoparticles for 4 h, 6 h, 12 h and 20 h. Histograms of cells after fluorescence-activated single cell sorting analysis are shown.

DAPI: 4′,6-diamidino-2-phenylindole; HA-PEG: Hyaluronic acid-poly(ethylene glycol); HA-PEI: Hyaluronic acid-polyethyleneimine; M2: Alternatively activated macrophage.