Figure 1.

KRAS p.G12C detection.

(A) Calibration curve. Observed frequencies showed consistent correlation with expected frequencies (R² = 0.997). Limit of detection (LOD) was 0.414 and limit of quantification (LOQ) was 1.255. (B) Dot-plot from the digital PCR (dPCR) assay. Positive detection of p.G12C variant with 13% mutant allele frequency (MAF): mutated alleles were recognised by FAM probe and correspond to blue data points, while wild-type alleles are detected by VIC probe and are displayed as red dots. Yellow dots represent empty wells (no amplification).