Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 6;40:312. doi: 10.1186/s13046-021-02122-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 5 — HOTAIRM1 promotes EGR1 degradation through MDM2-mediated ubiquitination. a Western blot analysis of EGR1 levels in the different transfection groups of OCI-AML3 and OCI-AML2 cells. b Relative EGR1 mRNA expression in HOTAIRM1-silenced and HOTAIRM1-overexpressing cells. c EGR1 protein expression in transfected OCI-AML3 and OCI-AML2 cells after treatment with MG132. d EGR1 expression in transfected OCI-AML3 and OCI-AML2 cells treated with CHX. e Western blot analysis of endogenous EGR1 ubiquitination in transfected OCI-AML3 and OCI-AML2 cells. f IP assays were used to investigate the binding of MDM2 to EGR1 in OCI-AML3 cells. g The level of EGR1 and MDM2 were examined by western blotting in OCI-AML3 and OCI-AML2 cells transduced with shMDM2 or transfected with the MDM2-Flag plasmid. h Western blot analysis of EGR1 ubiquitination in OCI-AML2 cells transfected with the MDM2-Flag plasmid. i The interactions between EGR1 or MDM2 and HOTAIRM1 were validated by RNA pulldown and subsequent western blotting. j The binding of MDM2 to EGR1 in HOTAIRM1-silenced and HOTAIRM1-overexpressing cells was investigated by IP assays. The data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. n.s. indicates no significant difference