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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: J Thromb Haemost. 2021 Jul 19;19(10):2480–2494. doi: 10.1111/jth.15440

Figure 3. Fibrinogen promotes tumor growth by altering expression of key genes involved in cell cycle regulation and metabolism.

Figure 3.

(A) MC38 cells were injected into the dorsal subcutis of Fib+ and Fib− mice and harvested 14 days after inoculation for RNAseq analyses. Note that at this early time point there were no fibrinogen-dependent differences in tumor size. (B) Volcano plot illustrating differentially expressed genes. Red dots are genes upregulated in tumors from Fib− mice relative to Fib+, and green dots are downregulated genes compared to Fib+. 14-3-3σ is one of the most significantly upregulated genes in Fib− tumors. Also shown are gene ontology (GO) analyses of biological functions for genes downregulated (C) and upregulated (D) in Fib− tumors relative to Fib+ tumors. Western blot (E) and relative densitometry (F) confirming increased expression of 14-3-3σ in Fib− tumors relative to Fib. Error bars represent SEMs (*p < 0.05, Mann Whitney U test).