Figure 1:

a. Diagnostic T1-weighted post-contrast MRI b. T2 post-contrast MRI at recurrence. c-f. PLNTY features in the primary tumor. Recurrent tumor shows abundant mitoses (g), microvascular proliferation (h), markedly elevated proliferative index (Ki-67, i), and pseudopalisading necrosis (k), without CD34 expression (i). Scale bar= 100 μm (c, g); 200 μm (d-f, h, i, k, l). j. Sequencing results m. DNA methylation-based classification results. T-SNE dimensionality reduction was performed on the primary (▼) and recurrent (▲) tumors and a subset of CNS tumors from the DKFZ reference set (color coded according to methylation cluster assignation) with the Rtsne package version 0.15. Analyses were performed using the mnp.v11b6 package in R version 3.6.1; as described by Capper et al.[3]