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SYBR Green-based PCR with primers targeting the mutated Spike del 69/70 genes in S-negative samples (N-gene is used as a positive control). Panel A show the amplification curves of the targeted regions in N, and Spike genes in the S-negative samples using three primer sets corresponding to N, Spike WT and Spike del 69/70. Panel B show the melting curves for the products amplified in S-negative samples by N and Spike del 69/70 primers only
