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List of primer sets
| Target gene | Primer name | Forward sequence (5′–3′) | Reverse sequence (5′–3′) |
|---|---|---|---|
| Spike | Spike WT | GGTTCCATGCTATACATGTCTC | GGTCTTCGAATCTAAAGTAGTACCA |
| Spike | Spike del 69/70 | GTTCCATGCTATCTCTGG | GGGACTGGGTCTTCGAATCT |
| NSP6 | NSP6 WT | GGTTGATACTAGTTTGTCTGGTTTT | AACGAGTGTCAAGACATTCATAAG |
| N | WH-NIC | CGTTTGGTGGACCCTCAGAT | CCCCACTGCGTTCTCCATT |
Deleted nucleotides are in bold and underlined
