Fig. 2. Heat maps of SWV optimization for (a) full width half maximum (FWHM), (b) peak current and (c) peak position, with meshed regions showing the preferred value ranges. SWV optimization was carried out by varying frequency from 100 to 500 Hz and amplitude from 0.01 to 0.1 V. The CRISPR/Cas system was optimized for the percentage difference between the treated and untreated electrode current as a function of (d) MgSO4 concentration, (e) trans-cleavage period, and (f) Cas–gRNA complex concentration (N = 3).
