Fig. 3. Synergistic antiviral activity of otamixaban combined with camostat and nafamostat. (a) Antiviral activity of otamixaban in combination with 1 nm camostat (green) or 0.1 nm nafamostat (orange) compared with otamixaban alone (grey) was analyzed using Calu-3 cells and VSV pseudotypes harboring either SARS-2-S (solid lines) or VSV-G (dashed lines) as described for Fig. 2b. Corresponding IC50 values are highlighted with dotted lines and annotated on the left. The average of three biological replicates ± SD is shown. (b) Combination index as a function of inhibition strength. The dashed line at CI = 1.0 indicates additivity (neither synergism nor antagonism); combination indices below the dotted line indicate synergism (CI < 1). Inhibition range with strong synergism (IC < 0.3) is marked by shaded areas.
