FIG. 6.
GA increases the association of Hsp90 with Gcn2 in vitro. Gcn2 was translated at 30°C in rabbit reticulocyte lysate. GA was added during synthesis at a concentration of 20 μM. The top sections represent the input, and the bottom sections represent the immune pellets after coimmunoprecipitation (IP) with the anti-Hsp90 monoclonal antibody. (A) Plasmids pYes/GSTGcn2, pYes/Gcn2, and pYes/Gcn2ΔN were used for in vitro translation; (B) plasmids pYes/Gcn2 and pYes/Gcn2K559V were used. The migration positions of the different Gcn2 variants are indicated with arrows.