Fluorescence interference assay. Compound 1 was isolated from a fluorescence based protease inhibitor assay and was then rescreened in dose response against the fluorophore alone (7-aminomethylcoumarin) and found not to interfere with fluorescence detection throughout the IC50 range of compound 1. Reprinted with permission from: T. D. Tran, B. A. P. Wilson, C. J. Henrich, L. M. Staudt, L. R. H. Krumpe, E. A. Smith, J. King, K. L. Wendt, A. M. Stchigel, A. N. Miller, R. H. Cichewicz, B. R. O’keefe and K. R. Gustafson, J. Nat. Prod., 2019, 82, 154–162. Copyright 2019 American Chemical Society.