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. 2021 Oct 1;35(19-20):1333–1338. doi: 10.1101/gad.348762.121

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© 2021 Shan et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 2. — DPP4+ APCs are the principal source of Il33 expression in iWAT. (A) mRNA levels of Il33 in freshly sorted APCs (n = 5). (B) IL-33 expression in cultured APCs. β-ACTIN levels were used as loading controls. Each lane represents one cell lysate derived from pooled cells from four mice. (C) mRNA levels of Il33 and individual Il33 isoforms (Il33a and Il33b) in freshly sorted DPP4+ APCs from iWAT of control and Pdgfrb-Il33^KO mice after doxycycline-containing chow diet feeding (n = 4). (D) Il33 expression in whole iWAT of control and Pdgfrb-Il33^KO mice after doxycycline-containing chow diet feeding (n = 8 for control; n = 9 for Pdgfrb-Il33^KO). (E) IL-33 levels in whole iWAT of control and Pdgfrb-Il33^KO mice after doxycycline-containing chow diet feeding. Bars represent mean + SEM. (***) P <0.001 by two-tailed unpaired Student's t-test.