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. 2021 Oct 1;35(19-20):1333–1338. doi: 10.1101/gad.348762.121

Figure 4.

Figure 4.

Isoproterenol induces Il33 expression in DPP4+ APCs via CREB. (A) ChIP-seq analysis of phosphorylated CREB (S133; p-CREB) occupancy at the Il33 locus in cultured DPP4+ APCs treated with isoproterenol or vehicle. Red boxes indicate bound regions (P1–P3) harboring predicted CREB response elements (“TGACGT/C”). Cells for ChIP-seq analysis were pooled from both iWAT depots of eight mice. (B) ChIP-PCR of pCREB occupancy at regions P1–P3 within cultured APCs following treatment with vehicle or isoproterenol. The cells were pooled from both iWAT depots of eight mice. (N.D.) Not detected. (C) CREB expression in DPP4+ APCs following CRISPR–Cas9-mediated inactivation of the Creb locus using independent gRNAs. Each lane represents lysates from cells pooled from both iWAT depots of four mice. (D) Il33 expression in cultured DPP4+ APCs following treatment with vehicle or isoproterenol. n = 4 for each group. Bars represent mean + SEM. (*) P< 0.05, (***) P < 0.001 by two-way ANOVA.