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. 2021 Oct 1;35(19-20):1356–1367. doi: 10.1101/gad.348667.121

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© 2021 Paiano et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 2. — Decreased DNA synthesis or Polα/primase activity increases resection. (A–C) Average reads per million (RPM) aggregated ±2.5 kb around the strongest 200 AsiSI break sites of END-seq (A), RPA ssDNA sequencing (SSDS) (B), and SAR-seq (C) in Lig4^−/− pre-B cells treated with or without 5 µM aphidicolin (APH) during AsiSI cutting. (D) Box plots of calculated resection lengths (left) and ratio of resected breaks versus total breaks (right) as detected by END-seq. Each data point represents a specific AsiSI genomic location. AsiSI cutting was induced in Lig4^−/− cells with either no additional treatment (NT), 5 µM APH, or 1 µM DNA Polα inhibitor (POLAi) concurrent treatment. (E,F) Box plots of signal intensity for RPA SSDS (E) and SAR-seq (F) in Lig4^−/− cells after AsiSI induction with either NT, 5 µM APH, or 1 µM POLAi concurrent treatment. All panels show representative data sets from two independent replicate experiments.