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. 2021 Oct 1;35(19-20):1368–1382. doi: 10.1101/gad.348581.121

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© 2021 Kawasumi et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 5. — The synthetic lethality of ctf18 ddx11 is rescued by WAPL depletion in vertebrate cells but not in budding yeast. (A) Growth curves of cells of indicated genotypes with or without auxin treatment as in Figure 2A. (B) Frequency of lagging chromosomes at anaphase as in Figure 3C. The results of two independent experiments (except for WT cells) are plotted. (C) Metaphase chromosomes were analyzed for sister chromatid cohesion as in Figure 3A, with an additional class termed “unseparated type” typical of the wapl cell shown. (D–G) Genetic interactions between ctf18Δ, chl1Δ, chl1-K48R, wpl1Δ and elg1Δ were assessed by tetrad dissection analysis of the indicated diploid budding yeast strains.