Figure 8.

Subclustering of muscle and intestinal cells separates them by position along the anterior–posterior body axis. (A) Peaks near genes that should be expressed throughout a tissue, like hlh-1 and myo-3 in body wall muscle or end-1 and elt-2 in the intestine, show accessibility in cells throughout the UMAP. (B) In both the muscle and intestine data, we can detect subclusters of cells that show peaks near genes that mark the anterior or posterior regions of these tissues based on literature and microscopy data (Packer et al. 2019).