Skip to main content
. 2021 Oct 6;7(41):eabj7487. doi: 10.1126/sciadv.abj7487

Fig. 7. Ribozyme-mediated RNA polymerization coupled to ribozyme-catalyzed GTP synthesis.

Fig. 7.

(A) Autoradiogram of polymerization products separated by denaturing 20% PAGE. The first reaction lacked GTP and GTR1 (No GTR1, black), the second reaction lacked GTP but contained the products from a GTP synthesis reaction with GTR1 (+ GTR1, blue), and the third reaction contained GTP (+ GTP, blue). For each of the three different reactions, samples from three polymerization time points were analyzed (0.5, 3, and 24 hours). The identity of the templated NTP is shown on the right for nucleotide (nt) additions +1 to +4. (B) Quantification of extension products. The fraction of primers extended by at least two nucleotides is plotted as a function of reaction time for three different conditions described in (A). Error bars are SDs from three experiments and mostly smaller than the symbols. The values at 24-hour polymerization time are 28.1 ± 1.1% (no GTR), 68.3 ± 2.0% (+ GTR), and 94.0 ± 0.9% (+ GTP).