Figure 7.

Identification of candidate genes responsible for close clustering of in vivo-grown and in vitro grown (IVG) oocytes. (A) Venn diagram of 6343 DEGs (red and gray; padj < 0.05) between in vivo-grown oocyte-like IVG (IVL) oocytes and in vivo-grown oocytes and 4121 DEGs (yellow and gray; padj < 0.05) between canonical 7%-IVG oocytes and IVL oocytes. The expression levels of 1623 genes (yellow) in IVL oocytes were restored to those in in vivo-grown oocytes. GO analysis of the 1623 DEGs showed enrichment of GO term 'regulation of transcription and DNA-templated'. Horizontal axis indicates −log₁₀(q-value). (B) Gene expression levels in IVL (gray) and in vivo-grown (white) oocytes relative to that in canonical-7%-IVG oocytes (black). (C) Correlation between the expression of Nobox and other genes [log₂(CPM+1)≥6]. White and gray dots indicate a correlation score ≥ 0.7 and < 0.7, respectively.