Figure 1.

NR supplementation does not counteract HFD-induced obesity. (A) Bodyweight during 8 weeks of chow or HFD feeding of B6N and B6J mice, (B) inguinal, (C) epididymal fat pad, (D) liver, (E) quadriceps, (F) tibialis anterior, and (G) soleus muscle weight. Data are presented as mean ± s.e.m., n = 10 HFD, n  =5 chow for body weight, n = 6 HFD, n = 5 chow for liver and fat weights, n = 3–5 for muscle weights. Tests for statistical significance were done using repeated three-way ANOVA, with time, diet, and mouse strain as factors (A) or ordinary two-way ANOVA with diet and mouse strain as factors followed by Tukey’s test for multiple comparisons. Data for NR vs no NR supplementation were analyzed together since no significant differences were found for NR vs no NR. ⁺P  < 0.05 for HFD vs chow, ^*P  < 0.05 for B6J vs B6N.