Figure 5.

Water intake and feeding frequency are altered by NR supplementation in HFD-fed mice. Caloric consumption (A and C) and water intake (B and D) as well as feeding frequency (C and F) as a measure for activity were determined by indirect calorimetry in metabolic cages. Upper panel: night/active phase, lower panel: day/resting phase. Data are presented as mean ± s.e.m., n = 4 for chow, n = 7 for B6N HFD, n = 10 for B6N HFD+NR, B6J HFD, and HFD+NR. Tests for statistical significance were done using ordinary two-way ANOVA with diet and mouse strain as factors followed by Tukey’s test for multiple comparisons. Data for NR vs no NR supplementation were analyzed together since no significant differences were found for NR vs no NR. ⁺P  < 0.05 for HFD vs chow, ^*P  < 0.05 for B6J vs B6N.