Figure 2.
Experimental Design. (A) Schematic of experimental design. (B) Timeline of experiments. Mice received no treatment following closed articular fracture of the knee or a single intra-articular (IA) injection of IL-1Ra immediately following fracture. At 7 days post-fracture, synovial fluid (SF) collected and pooled from 3 fractured and 3 control limbs per group, and whole blood (WB) collected for n=3 per group. At 14 days post-fracture, synovial fluid collected and pooled from 4 fractured limbs for n=2 per group, and whole blood (WB) collected for n=8 per group. Hind limbs collected from all mice for microCT and histology.