Figure 5. Fast sustained release with very weak Ca²⁺-dependence.

(A) Examples of a capacitance trace showing a sustained component of release. (B) Plot of the number of vesicles released between 10 and 100 ms as estimated from capacitance measurements divided by the time interval (90 ms) versus the post-flash Ca²⁺ concentration (n = 71 cMFBs). The line represents a linear fit to the data with a slope of 6 vesicles ms⁻¹ µM⁻¹ (Pearson correlation coefficient = 0.06, r² = 0.003; P_{Pearson correlation} = 0.6). (C) Plot of the number of vesicles released between 10 and 100 ms as estimated from deconvolution analysis divided by the time interval (90 ms) versus the post-flash Ca²⁺ concentration (n = 51 cMFB-GC pairs). The line represents a linear fit to the data with a slope of 10 vesicles ms⁻¹ µM⁻¹ (Pearson correlation coefficient = 0.3, r² = 0.1; P_{Pearson correlation} = 0.01).