Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Chemical compound, drug | NaCl | Sigma-Aldrich | Cat. # S9888 | |
Chemical compound, drug | NaHCO3 | Sigma-Aldrich | Cat. # S6297 | |
Chemical compound, drug | Glucose | Sigma-Aldrich | Cat. # G8270 | |
Chemical compound, drug | AP 5 | Sigma-Aldrich | Cat. # A78403 | |
Chemical compound, drug | KCl | Sigma-Aldrich | Cat. # P9333 | |
Chemical compound, drug | CaCl2 | Sigma-Aldrich | Cat. # C5080 | For extracellular solution |
Chemical compound, drug | CaCl2 | Sigma-Aldrich | Cat. # 21115 | For intracellular solution |
Chemical compound, drug | EGTA | Sigma-Aldrich | Cat. # E0396 | |
Chemical compound, drug | NaH2PO4 | Merck | Cat. # 106342 | |
Chemical compound, drug | Tetrodotoxin | Tocris | Cat. # 1078 | |
Chemical compound, drug | MgCl2 | Sigma-Aldrich | Cat. # M2670 | |
Chemical compound, drug | TEA-Cl | Sigma-Aldrich | Cat. # T2265 | |
Chemical compound, drug | HEPES | Sigma-Aldrich | Cat. # H3375 | |
Chemical compound, drug | NaGTP | Sigma-Aldrich | Cat. # G8877 | |
Chemical compound, drug | Na2ATP | Sigma-Aldrich | Cat. # A2383 | |
Chemical compound, drug | DMnitrophen | Synptic systems | Cat. # 510016 | |
Chemical compound, drug | CsOH | Sigma-Aldrich | Cat. # C8518 | |
Chemical compound, drug | Atto594 | ATTO-TEC | Cat. # AD 594 | |
Chemical compound, drug | OGB1 | Thermo Fisher Scientific | Cat. # 06806 | |
Chemical compound, drug | OGB-5N | Thermo Fisher Scientific | Cat. # 944034 | |
Chemical compound, drug | Fluo-5F | Thermo Fisher Scientific | Cat. # F14221 | |
Chemical compound, drug | KOH solution | Roth | Cat. # K017.1 | |
Chemical compound, drug | Kynurenic acid | Sigma-Aldrich | Cat. # K3375 | |
Chemical compound, drug | Cyclothiazide | Sigma-Aldrich | Cat. # C9847 | |
Chemical compound, drug | Ca2+ Calibration Buffer Kit | Thermo Fisher Scientific | Cat. # C3008MP | |
Chemical compound, drug | Caged fluorescein | Sigma-Aldrich | Cat. # F7103 | |
Chemical compound, drug | Glycerol | Sigma-Aldrich | Cat. # G5516 | |
Chemical compound, drug | Isoflourane | Baxter | Cat. # Hdg9623 | |
Chemical compound, drug | Aqua B. Braun | Braun | Cat. # 00882479E | For extracellular solution |
Chemical compound, drug | Sterile Water | Sigma-Aldrich | W Cat. # 3500 | For intracellular solution |
Strain, strain background (mouse C57BL/6N) | Female, male C57BL/6N |
Charles river | https://www.criver.com/ | |
Other | Vibratome | LEICA VT 1200 | https://www.leica-microsystems.com/ | |
Other | Femto2D laser-scanning microscope | Femtonics | https://femtonics.eu/ | |
Other | UV laser source | Rapp OptoElectronic | https://rapp-opto.com/ | 375 nm, 200 mW |
Other | DMZ Zeitz Puller | Zeitz | https://www.zeitz-puller.com/ | |
Other | Borocilicate glass | Science Products | https://science-products.com/en/ | GB200F-10 With filament |
Other | HEKA EPC10/2 amplifier | HEKA Elektronik | https://www.heka.com/ | |
Other | Ti:Sapphire laser | MaiTai, SpectraPhysics | https://www.spectra-physics.com/ | |
Other | Ca2+ sensitive electrode (ELIT 8041 PVC membrane) | NICO 2000 | http://www.nico2000.net/index.htm | |
Other | Single junction silver chloride reference electrode (ELIT 001 n) | NICO 2000 | http://www.nico2000.net/index.htm | |
Other | PH/Voltmeter | Metler toledo | https://www.mt.com/de/en/home.html | |
Other | Osmomat 3000 | Gonotec | http://www.gonotec.com/de | |
Other | TC-324B perfusion heat controller | Warner Instruments | https://www.warneronline.com/ | |
Software, algorithm | MES | Femtonics | https://femtonics.eu/ | |
Software, algorithm | Igor Pro | Wavemetrics | https://www.wavemetrics.com/ | |
Software, algorithm | Patchmaster | HEKA Elektronik | https://www.heka.com/ | |
Software, algorithm | Adobe illustrator | Adobe | https://www.adobe.com/products/illustrator.html | |
Software, algorithm | Mathematica | Wolfram | https://www.wolfram.com/mathematica/ | |
Software, algorithm | Maxchelator | Stanford University | https://somapp.ucdmc.ucdavis.edu/pharmacology/bers/maxchelator/ |