Table 1. Parameters for weak, middle, and strong post-flash Ca2+ elevations.
weak Ca2+ elevation | middle Ca2+ elevation | strong Ca2+ elevation | |
---|---|---|---|
UV illumination | |||
Duration (ms) | 0.1 or 1 | 0.1 | 0.1 or 0.2 |
Intensity (%) | 10–100 | 20–100 | 100 |
Concentration in intracellular solution (mM) | |||
ATTO 594 | 0.010 | 0.020 | 0.020 |
Fluo 5F | 0.050 | 0 | 0 |
OGB 5N | 0 | 0.200 | 0.200 |
CaCl2 | 0.500 | 2.000 | 10.000 |
DM-N | 0.500 | 2.000 | 10.000 |
Obtained peak post-flash Ca2+ (µM) | |||
Min | 1.1 | 2.7 | 15.7 |
Max | 7.1 | 36.0 | 62.6 |
Median | 2.4 | 8.8 | 25.1 |
Simulated uncaging fraction of DMn | |||
α | 0.08–0.5 | 0.15–0.55 | 0.14–0.25 |