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. 2021 Oct 6;10:e70408. doi: 10.7554/eLife.70408

Table 1. Parameters for weak, middle, and strong post-flash Ca2+ elevations.

weak Ca2+ elevation middle Ca2+ elevation strong Ca2+ elevation
UV illumination
 Duration (ms) 0.1 or 1 0.1 0.1 or 0.2
 Intensity (%) 10–100 20–100 100
Concentration in intracellular solution (mM)
 ATTO 594 0.010 0.020 0.020
 Fluo 5F 0.050 0 0
 OGB 5N 0 0.200 0.200
 CaCl2 0.500 2.000 10.000
 DM-N 0.500 2.000 10.000
Obtained peak post-flash Ca2+ (µM)
 Min 1.1 2.7 15.7
 Max 7.1 36.0 62.6
 Median 2.4 8.8 25.1
Simulated uncaging fraction of DMn
 α 0.08–0.5 0.15–0.55 0.14–0.25