Table 2. Parameters for simulations of Ca²⁺ release from DMN cage.

Parameters		Values	References number / Notes
Resting Ca2+	[Ca2+]rest	227*10−9 M	Measured
Total magnesium	[Mg2+]T	0.5*10−3 M	Pipette concentration
Fluo-5F	[Fluo]	0 or 50 *10−6 M (see Table 1)	Pipette concentration
	K D	0.83 *10−6 M	Delvendahl et al., 2015
	k off	249 s-1	ibid
	k on	3*108 M−1s−1	Yasuda et al., 2004
OGB-5N	[OGB]	0 or 200*10−6 M (see Table 1)	Pipette concentration
	K D	31.4*10−6 M	Measured (Figure 3—figure supplement 1A)
	koff	6000 s−1	ibid.
	kon	2.5*108 M−1s−1	DiGregorio and Vergara, 1997
ATP	[ATP]	5 *10−3 M	Pipette concentration
Ca2+ binding	K D	2*10−4 M	Meinrenken et al., 2002
	k off	100 000 s−1	ibid.
	k on	5*108 M−1s−1	ibid.
Mg2+ binding	K D	100*10−6 M	Bollmann et al., 2000; MaxC
	k off	1000 s−1	ibid.
	k on	1*107 M−1s−1	ibid.
Endogenous buffer	[EB]	480 *10−6 M	Delvendahl et al., 2015
	K D	32*10−6 M	ibid
	k off	16 000 s−1	ibid.
	k on	5*108 M−1s−1	ibid.
Total DM nitrophen	[DMn]T	500*10−6 – 10*10−3 M (see Table 1)	Pipette concentration
Ca2+ binding	K D	6.5*10−9 M	Faas et al., 2005
	k off	0.19 s−1	ibid.
	k on	2.9*107 M−1s−1	ibid.
Mg2+ binding	K D	1.5*10−6 M	ibid.
	k off	0.2 s−1	ibid.
Uncaging fraction	α	See Table 1
Fast uncaging fraction	af	0.67	Faas et al., 2005
Photoproduct 1	[PP1]
Ca2+ binding	K D	2.38*10−3 M	Faas et al., 2005
	k off	69 000 s−1	ibid.
	k on	2.9*107 M−1s−1	ibid.
Mg2+ binding	K D	1.5*10−6 M	ibid.
	k off	300 s−1	ibid.
	k on	1.3*105 M−1s−1	ibid.
Photoproduct 2	[PP2]
Ca2+ binding	K D	124.1*10−6 M	Ibid.
	k off	3600 s−1	ibid.
	k on	2.9*107 M−1s−1	ibid.
Mg2+ binding	K D	1.5*10−6 M	ibid.
	k off	300 s−1	ibid.
	k on	1.3*105 M−1s−1	ibid.